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CD68 Mouse Recombinant Monoclonal Antibody
SKU: orb1520128
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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC |
|---|---|
| Dilution Range | Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 50% methanol. 2 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1 |
| Clone No. | KP-1 |
| Immunogen | Lysosomal fraction of human lung macrophages |
| Target | CD68 |
| Purity | Recombinant antibody was purified from cell culture supernatant |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 50 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD antigen CD68; CD68 antigen; GP110; LAMP4; macrophage antigen CD68; macrosialin; SCARD1; scavenger receptor class D, member 1
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Detection of human CD68 in FFPE tonsil by IHC. Antibody: Mouse monoclonal anti-CD68. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: DAB
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Detection of human CD68 in FFPE seminoma by IHC. Antibody: Mouse monoclonal anti-CD68. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: DAB. Counterstain:Hematoxylin (blue)
Detection of human CD68 (green) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Mouse anti-CD68 monoclonal antibody used at 1:500. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal™ . Counterstain: DAPI (blue).
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UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001242.2 |
|---|
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CD68 Mouse Recombinant Monoclonal Antibody (orb1520128)
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