You have no items in your shopping cart.
Mouse Cytokeratin 14 Recombinant Monoclonal Antibody
SKU: orb1806434
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FC, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 20 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG2a |
| Clone No. | LL002 |
| Immunogen | Human Cytokeratin 14 (C terminal) |
| Target | Cytokeratin 14 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 100 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CK14;CK-14;cytokeratin 14;Cytokeratin-14;EBS3;EBS4;K14;keratin 14, type I;keratin, type I cytoskeletal 14;Keratin-14;NFJ
Similar Products
−
Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody (RBITC) [orb2355738]
IF
Human, Mouse, Rat
Rabbit
Recombinant
RBITC
100 μlCytokeratin 14 Recombinant Rabbit Monoclonal Antibody (PerCP-Cy5.5) [orb2355739]
IF
Human, Mouse, Rat
Rabbit
Recombinant
PerCP/Cy5.5
100 μlCytokeratin 14 Recombinant Rabbit Monoclonal Antibody (PerCP) [orb2355740]
IF
Human, Mouse, Rat
Rabbit
Recombinant
PerCP
100 μlCytokeratin 14 Recombinant Rabbit Monoclonal Antibody (PE) [orb2355741]
IF
Human, Mouse, Rat
Rabbit
Recombinant
PE
100 μlCytokeratin 14 Recombinant Rabbit Monoclonal Antibody (FITC) [orb2355742]
IF
Human, Mouse, Rat
Rabbit
Recombinant
FITC
100 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Detection of human Cytokeratin 14 by western blot. Samples: Whole cell lysate (25 µg) from A-431, HCT 116, MCF-10A, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human Cytokeratin 14 by immunohistochemistry. Sample: FFPE section of bladder carcinoma. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434). Secondary: HRP-conjugated goat anti-mouse IgG.
Detection of human Cytokeratin 14 (shaded) in FaDu cells by flow cytometry. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-mouse IgG.
Detection of human Cytokeratin 14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434) used for IP at 20 µl/mg lysate.
Detection of human Cytokeratin 14 by immunocytochemistry. Sample: FFPE section of CAL-27 cells. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434). Secondary:HRP-conjugated goat anti-mouse IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000517.3 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Mouse Cytokeratin 14 Recombinant Monoclonal Antibody (orb1806434)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days