Mouse anti-Cytokeratin Monoclonal Antibody

SKU: orb1520125

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Description

Cytokeratin Antibody

Images & Validation

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Tested Applications	ICC, IF, IHC
Dilution range	Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. ICC-IF1:100 - 1:500. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Clone No.	AE1/AE3
Immunogen	Human epidermal callus
Target	Cytokeratin
Purity	The antibody was purified from cell culture supernatant
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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K10; Keratin-10; keratin, type I cytoskeletal 10; CK10; keratin 10, type I; KPP; Cytokeratin-10; CK-10; BIE; BCIE; cytokeratin 10; EHK; EBS4; NFJ; Keratin-14; keratin, type I cytoskeletal 14; K14; EBS3; Cytokeratin-14; cytokeratin 14; CK-14; CK14; keratin 14, type I; keratin, type I cytoskeletal 15; Cytokeratin-15; type I cytoskeletal 15; keratin-15, beta; keratin-15, basic; Keratin-15; CK15; K15; cytokeratin 15; CK-15; K1CO; keratin 15, type I; Keratin-16; PC1; KRT16A; keratin, type I cytoskeletal 16; keratin 16, type I; K1CP; focal non-epidermolytic palmoplantar keratoderma; FNEPPK; Cytokeratin-16; cytokeratin 16; CK-16; CK16; K16; NEPPK; K19; keratin, type I, 40-kd; keratin, type I cytoskeletal 19; K1CS; Keratin-19; Cytokeratin-19; cytokeratin 19; CK-19; CK19; 40-kDa keratin intermediate filament; keratin 19, type I

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Mouse anti-Cytokeratin Monoclonal Antibody

Detection of human Cytokeratin by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Mouse anti-Cytokeratin monoclonal antibody [AE1/AE3]. Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human Cytokeratin by immunocytochemistry. Sample: FFPE section of MCF7 cells. Antibody: Mouse anti-Cytokeratin monoclonal antibody [AE1/AE3] (orb1520125). Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human Cytokeratin by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Mouse monoclonal anti-Cytokeratin antibody [AE1/AE1] (orb1520125) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG. Counterstain: DAPI.

Detection of human Cytokeratin (green) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Mouse anti-Cytokeratin monoclonal antibody used at 1:500. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

Cytokeratin

UniProt

P13645

RefSeq (Protein):NP_000517, NP_002266, NP_000412, NP_005548, NP_002267

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000517, NP_002266, NP_000412, NP_005548, NP_002267

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Protocol Information

IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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