Mouse p16INK4a Recombinant Monoclonal Antibody

SKU: orb1806437

Description

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 4

Tested Applications	FC, ICC, IHC, WB
Dilution Range	Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : WB , ApplicationDilutionRange : 1:1000
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	IgG1
Clone No.	BC42
Immunogen	Human p16 INK4A
Target	p16INK4a
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	100 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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alternative reading frame;ARF;CDK4 inhibitor p16-INK4;CDK4I;CDKN2;cell cycle negative regulator beta;CMM2;cyclin-dependent kinase 4 inhibitor A;cyclin-dependent kinase inhibitor 2A;cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4);INK4;INK4A;MLM;MTS1;MTS-1;multiple tumor suppressor 1;P14;P14ARF;P16;P16INK4;p16-INK4;P16INK4A;P16-INK4A;P19;P19ARF;TP16

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Mouse p16INK4a Recombinant Monoclonal Antibody

Detection of human p16INK4a by western blot. Samples: Whole cell lysate (50 µg) from SK-MEL-28, HeLa, OVCAR-3, and OVCAR-8 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody [BC42] (orb1806437) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human p16INK4a by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Mouse anti-p16INK4a recombinant monoclonal antibody [BC42] (orb1806437). Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human p16INK4a (shaded) in HeLa cells by flow cytometry. Antibody: Mouse anti-p16INK4a recombinant monoclonal antibody [BC42] (orb1806437) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-mouse IgG.

Detection of human p16INK4a by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Mouse anti-p16INK4a recombinant monoclonal antibody [BC42] (orb1806437). Secondary: HRP-conjugated goat anti-mouse IgG.

Quick Database Links

Gene Symbol

p16INK4a

UniProt

P42771

RefSeq (Protein):NP_478104.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_478104.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ICC

Immunocytochemistry

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