Mouse Beta-Crosslaps (bCTx) ELISA Kit

SKU: orb775409

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Beta-Crosslaps(bCTx) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-Crosslaps(bCTx).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse bCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bCTx in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma, urine and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	78.13-5000 pg/mL
Sensitivity	27.5 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Beta CTx,b-CTx ELISA Kit, bCTXI ELISA Kit, bCTX-I ELISA Kit, B-Cr ELISA Kit, BCL ELISA Kit, Type I Collagen C-Telopeptide-Related Fraction ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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