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Mouse CCR6(Chemokine C-C-Motif Receptor 6) Microsample ELISA Kit

SKU: orb2997641
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Description

The Mouse CCR6(Chemokine C-C-Motif Receptor 6) Microsample ELISA Kit is designed to quantify CCR6(Chemokine C-C-Motif Receptor 6) Microsample in mouse tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.054 ng/mL.

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CCR6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CCR6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CCR6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CCR6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityMouse
Sample TypesTissue homogenates, cell lysates and other biological fluids.
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.054 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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CD196; BN-1; CKR-L3; CKR6; CKRL3; CMKBR6; DCR2; DRY-6; GPR-CY4; GPR29; GPRCY4; STRL22; Chemokine receptor-like 3; LARC receptor; G-protein coupled receptor 29
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Quality Guarantee

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Quick Database Links

UniProt
O54689

UniProt Details

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No UniProt data available

Protocol Information

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48 T
$ 330.00
96 T
$ 470.00
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