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Mouse CD33 protein

SKU: orb391408

Description

Recombinant mouse CD33 protein is produced by our mammalian expression system and the target gene encoding Asp18-Glu240 is expressed with a 6His tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
TargetCD33
ReactivityMouse
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceDLEFQLVAPE SVTVEEGLCV HVPCSVFYPS IKLTLGPVTG SWLRKGVSLH EDSPVATSDP RQLVQKATQG RFQLLGDPQK HDCSLFIRDA QKNDTGMYFF RVVREPFVRY SYKKSQLSLH VTSLSRTPDI IIPGTLEAGY PSNLTCSVPW ACEQGTPPTF SWMSTALTSL SSRTTDSSVL TFTPQPQDHG TKLTCLVTFS GAGVTVERTI QLNVTRKSGQ MREVDHHHHH H

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD33

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Mouse CD33 protein (orb391408)

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Available Sizes

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10 μg
$ 270.00
50 μg
$ 650.00
500 μg
$ 3,100.00
DispatchUsually dispatched within 5-10 working days
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