Recombinant Mouse Siglec-15 Protein

SKU: orb2994019

Description

Recombinant Mouse Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Arg24-Thr262 is expressed with a 6His tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Mouse
Biological Activity	BLI: Loaded Anti-Human Siglec15 mAb-mFc(Cat#orb2993156) on AMQ Biosensor, can bind Mouse Siglec-15-His(Cat#orb2994019) with an affinity constant of 0.33 nM as determined in BLI assay.
Tag	C-6His
Molecular Weight	Predicted: 26.5 KDa. Observed: 30-40 KDa, reducing conditions
Expression Region	Arg24-Thr262
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, 150mM NaCl, 5% Thehalose, 0.3% Chaps, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3

Human SIGLEC15 Protein, mFc-His Tag [orb689444]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 53.6 kDa after removal of the signal peptide.The apparent molecular mass of SIGLEC15-mFc-His is approximately 55-70 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 10 μg, 100 μg, 50 μg

Recombinant Mouse SIGLEC15 Protein, N-His [orb2963811]
Applications:
ELISA, SDS-PAGE, WB
Purity:
>90% as determined by SDS-PAGE.
MW:
25.08 kDa
Sizes Available: 1 mg, 50 μg, 100 μg
Recombinant Mouse SIGLEC15 Protein, N-His [orb2831534]
Purity:
>90% as determined by SDS-PAGE.
MW:
25.08 kDa
Sizes Available: 100 μg, 20 μg, 50 μg
Mouse SIGLEC-15 protein [orb867406]
Sizes Available: 50 μg, 500 μg, 1 mg

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UniProt

A7E1W8

UniProt Details

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No UniProt data available

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