Mouse Complement Component 5a (C5a) ELISA Kit

SKU: orb1806798

Featured

Description

Research Area

Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

−

Item 1 of 1

Application Notes

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C5a. You can calculate the concentration of Mouse C5a in the samples by comparing the OD of the samples to the standard curve.

Key Properties

−

Reactivity	Mouse
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Sandwich
Assay Time	3.5H
Range	62.5-4000pg/mL
Sensitivity	37.5 pg/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

C5a

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Mouse Complement Component 5a (C5a) ELISA Kit

Quick Database Links

Gene Symbol

C5a

UniProt

P06684

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

Find More Protocols