Mouse G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit

SKU: orb780776

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Estrogen Receptor 1(GPER).

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GPER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GPER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GPER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GPER in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.053 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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GPR30 ELISA Kit, CMKRL2 ELISA Kit, DRY12 ELISA Kit, FEG-1 ELISA Kit, GPCR-Br ELISA Kit, LERGU ELISA Kit, LyGPR ELISA Kit, Membrane estrogen receptor ELISA Kit, Chemoattractant receptor-like 2 ELISA Kit, Flow-induced endothelial G-protein coupled receptor 1 ELISA Kit

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Mouse G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit

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UniProt

Q8BMP4

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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