Mouse Growth Differentiation Factor 2 (GDF2) ELISA Kit

SKU: orb777570

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 2(GDF2).

Research Area

Immunology & Inflammation, Metabolism Research, Musculoskeletal & Connective Tissue Research, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GDF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GDF2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	27 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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BMP9 ELISA Kit, Bone Morphogenetic Protein 9 ELISA Kit

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Mouse Growth Differentiation Factor 2 (GDF2) ELISA Kit

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UniProt

Q9WV56

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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