Mouse IGF-I R Protein, His Tag

SKU: orb511248

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Description

Mouse IGF-I R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1).

Images & Validation

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Application Notes

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.7 kDa and 24.3 kDa respectively. The protein migrates as 45-55 kDa (partial β chain), 100-125 kDa (α chain) and 150 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	IGF-I R / IGF-1 R
Reactivity	Mouse
Tag	C-10×His
Molecular Weight	81.7 kDa, 24.3 kDa
Expression Region	Glu 31 - His 936
Purity	90%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Mouse IGF-I R / CD221 Protein.

SDS-Page analysis of Mouse IGF-I R / CD221 Protein.

Immobilized Mouse IGF-I R, His Tag (Cat. No. orb511248) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. orb257564) with a linear range of 7.8-125 ng/mL (QC tested).

Quick Database Links

Gene Symbol

IGF-I R / IGF-1 R

UniProt

Q60751

UniProt Details

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No UniProt data available

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