Mouse IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

SKU: orb347504

Description

Mouse IgG (H&L) antibody (TRITC)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, FC, FLISA, IF
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Mouse
Application Notes	Anti-Mouse IgG Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Mouse IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
Conjugation	TRITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Anti-Mouse IgG Antibody rhodamine Conjugation, Rabbit Anti-Mouse IgG Antibody rhodamine Conjugated, Rabbit Anti-Mouse IgG TRITC Conjugated Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Mouse IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Dot Blot of Anti Mouse IgG (Rabbit) mx Hu-Rhodamine conjugated. Lane 1: 100 ng of Ms IgG. Lane 2-5: serial dilution 3 fold starting at 100 ng. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Mouse IgG mx Hu-Rhodamine Conj'd at 1 mg/ml at RT for 30 minutes. Block: orb348637 at RT for 30 minutes.

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Flow Cytometry

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Immunofluorescence

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DOT

Dot Blot

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