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Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated

SKU: orb347378

Description

Mouse IgG IgA IgM (H&L) antibody (FITC)

Images & Validation

Tested ApplicationsDOT, FC, FLISA, IF
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
ReactivityMouse
Application Notes
Anti-Mouse IgG IgA IgM Fluorescein Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenMouse IgG IgA and IgM whole molecule
PurityThis product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. This reagent is suitable for the detection of all mouse isotypes and chain combinations.
ConjugationFITC

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration2.0 mg/mL
DisclaimerFor research use only

Alternative Names

Goat anti-mouse IgG IgA IgM antibody fluorescein conjugation, goat anti-mouse IgGAM FITC conjugated antibody

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Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated

Dot Blot for Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated. Lane 1A: 100 ng Ms IgG. Lane 1B: 100 ng Ms IgA. Lane 1C: 100 ng Ms IgM. Lanes 2-5: serial dilution 3 fold of respective Antigen. Primary Antibody: none. Secondary Antibody: Goat Anti-Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated at 1:1000 dilution at RT for 1 hour. Block: orb348637 at RT for 30 minutes.

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Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
DOT
Dot Blot
View Protocol

Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated (orb347378)

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2 mg
$ 460.00
DispatchUsually dispatched within 5-10 working days
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