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Mouse IgG + IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
SKU: orb347384
Description
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:40,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Mouse IgG and IgM whole molecule was produced by repeated immunization with Mouse IgG and IgM whole molecule in goat. |
| Purity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against No reaction was observed against bovine, horse or human serum proteins. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Mouse IgG and IgM whole molecule Antibody, Gt-a-Mouse IgG and IgM whole molecule Peroxidase conjugated, Mouse IgG and IgM whole molecule Peroxidase Antibody in goat, Mouse IgG and IgM whole molecules peroxidase conjugated Secondary Antibody.
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ELISA Results of Goat Anti-Mouse IgG + IgM Antibody Peroxidase Conjugated Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins tested against purified Mouse IgG + IgM. Each well was coated in duplicate with 10 µg of Mouse IgG (p/n orb2652749) [Red Line], Mouse IgM (IgG/IgA free) [Green Line], and Mouse IgG/IgM (p/n orb2652749/orb346283) [Blue Line]. The working dilution is 1:175000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, and TMB substrate (p/n orb348651).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Mouse IgG + IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed (orb347384)
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