Mouse IgM (H&L) Antibody Peroxidase Conjugated

SKU: orb346680

Description

Mouse IgM (H&L) antibody (Peroxidase)

Images & Validation

−

Item 1 of 1

Tested Applications	DOT, ELISA, IHC, WB
Dilution range	ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Reactivity	Mouse
Application Notes	This secondary peroxidase conjugated antibody anti-Mouse has been tested by dot blot and ELISA and is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Mouse IgM (H&L) peroxidase conjugated antibody has been assayed against 1.0 ug of mouse IgM in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product. Read more...

Key Properties

−

Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Mouse IgM (H&L) was produced by repeated immunization with mouse IgM whole molecule in rabbit.
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. This reagent primarily recognizes Mouse IgM however some light chain cross reactivity will occur against other mouse immunoglobulins.
Conjugation	HRP

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	10 mg/mL
Disclaimer	For research use only

Alternative Names

−

Mouse IgM (H&L) Antibody, Rb-a-Mouse IgM (H&L) Peroxidase conjugated, Mouse IgM (H&L) Peroxidase Antibody in rabbit, Rabbit peroxidase conjugated Secondary Antibody, rabbit anti-Mouse IgM HRP Conjugated Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Mouse IgM (H&L) Antibody Peroxidase Conjugated

Dot Blot results of Rabbit Anti-Mouse IgM Antibody Peroxidase Conjugated. Dots are Mouse IgM at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: none. Secondary Antibody: Rabbit anti-Mouse IgM-HRP at 1 µg/ml for 1hr at RT.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

DOT

Dot Blot

View Protocol

Find More Protocols