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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Tag | N-6His |
| Expression Region | Asn36-Ser192 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 19.7 KDa. Observed: 20 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 50mM NaCl, 0.1mM EDTA, 0.05% Tween 80, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-18; Il18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; Igif
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Protocol Information
Recombinant Mouse IL-18 Protein (orb3002400)
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