Mouse IL 20 Protein

SKU: orb426488

This product can be custom made

Description

Recombinant of mouse IL 20 protein

Images & Validation

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Application Notes	Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.552 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-20 as a Reference Standard

Key Properties

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Source	Escherichia Coli
Biological Activity	The biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100ng/mL of Interleukin-20 recombinant mouse, corresponding to a specific activity of 10,000 units/mg of Interleukin-20 recombinant mouse.
Purity	Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Protein Sequence	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Lys-Thr-Leu

Storage & Handling

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Storage	Stability: Lyophilized Interleukin-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL20 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
Form/Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Buffer/Preservatives	Lyophilized from a concentrated (1mg/ml) solution containing no additives.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL10D, ZCYTO10, IL-20, MGC96907, Four alpha helix cytokine Zcyto10.

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