Mouse IL1RA protein

SKU: orb391953

Description

Recombinant mouse IL1RA protein is produced by our E. coli expression system and the target gene encoding Arg27-Gln178 is expressed.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Target	IL1RN
Reactivity	Mouse
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Protein Sequence	MRPSGKRPCK MQAFRIWDTN QKTFYLRNNQ LIAGYLQGPN IKLEEKIDMV PIDLHSVFLG IHGGKLCLSC AKSGDDIKLQ LEEVNITDLS KNKEEDKRFT FIRSEKGPTT SFESAACPGW FLCTTLEADR PVSLTNTPEE PLIVTKFYFQ EDQ

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL1F3; IL1RA; Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 inhibitor; Anakinra

Human IL1RA Protein, mFc Tag [orb2321379]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 62.4 kDa after removal of the signal peptide. The apparent molecular mass of IL1RA-mFc is approximately 70-100 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 50 μg, 10 μg, 100 μg

Item 1 of 1
Mouse IL1RA ELISA Kit [orb391096]
Reactivity:
Mouse
Dynamic Range:
156 pg/ml - 10000 pg/ml
Sensitivity:
80 pg/ml
Sizes Available: 96 T

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Quick Database Links

Gene Symbol

IL1RN

UniProt

P25085

NCBI Gene ID

16181

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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