Mouse Integrin Alpha 3 (ITGa3) ELISA Kit

SKU: orb777526

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 3(ITGa3).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGa3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGa3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGa3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.115 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD49c ELISA Kit, CD49-C ELISA Kit, ITG-A3 ELISA Kit, GAP-B3 ELISA Kit, GAPB3 ELISA Kit, MSK18 ELISA Kit, VCA-2 ELISA Kit, VL3A ELISA Kit, VLA3a ELISA Kit, FRP-2 ELISA Kit, Galactoprotein B3 ELISA Kit, Alpha 3 Subunit Of VLA-3 Receptor ELISA Kit, CD49 antigen-like family member C ELISA Kit

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Mouse Integrin Alpha 3 (ITGa3) ELISA Kit

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UniProt

Q62470

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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