Mouse Interleukin 5 Receptor Alpha (IL5Ra) ELISA Kit

SKU: orb777578

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 Receptor Alpha(IL5Ra).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL5Ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL5Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL5Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL5Ra in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	29.9 pg/mL
Concentration	4000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CDw125 ELISA Kit, CD125 ELISA Kit, IL5R-A ELISA Kit, IL5-RA ELISA Kit, HSIL5R3 ELISA Kit, IL5R ELISA Kit, IL5R-A ELISA Kit

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Mouse Interleukin 5 Receptor Alpha (IL5Ra) ELISA Kit

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UniProt

P21183

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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