Mouse Kallikrein 1 (KLK1) ELISA Kit

SKU: orb2667699

Description

Images & Validation

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Application Notes

standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse KLK1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Sample Types	Serum, plasma and other biological fluids.
Assay Type	Competitive
Assay Time	2.5h
Range	0.32-20 ng/mL
Sensitivity	0.14 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein

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UniProt

P15947

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No UniProt data available

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