Mouse Kallikrein 6 (KLK6) ELISA Kit

SKU: orb778553

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 6(KLK6).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KLK6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLK6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KLK6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLK6 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, cerebrospinal fluid, breast milk and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	13.5 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ZYME ELISA Kit, Bssp ELISA Kit, Klk7 ELISA Kit, Neurosin,Zyme ELISA Kit, PRSS18 ELISA Kit, PRSS9 ELISA Kit, SP59 ELISA Kit, HK6 ELISA Kit, Protease M ELISA Kit, Serine protease 18 ELISA Kit

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UniProt

Q91Y82

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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