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Mouse Lipopolysaccharide (LPS) ELISA Kit

SKU: orb776857
Free Kit Promotion

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipopolysaccharide(LPS).

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA
Application Notes
standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LPS in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityMouse
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.051 ng/mL
Concentration10 ng/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

LOS ELISA Kit, Lipoglycans ELISA Kit, Lipooligosaccharide ELISA Kit, Lipo-Oligosaccharide ELISA Kit, Endotoxin ELISA Kit

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Mouse Lipopolysaccharide (LPS) ELISA Kit

Standard curve for orb776857 ELISA kit.

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Product Information
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Manual
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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Mouse Lipopolysaccharide (LPS) ELISA Kit (orb776857)

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Applications: ELISA|Reactivity: Mouse

该试剂盒采用成熟稳定的夹心ELISA检测体系,具有较高的特异性、重复性和检测稳定性,适用于小鼠样本中LPS水平的定量分析。相较于微量样本版本,其检测灵敏度更高、低浓度样本检测能力更强,尤其适用于需要准确评估微小浓度变化的实验研究。此外,其样本适用范围广、操作流程规范,能够满足基础研究及相关机制研究中对LPS检测的需求。

- 蔡思敏上海药物研究所
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Applications: ELISA|Reactivity: Mouse

该试剂盒采用双抗体夹心ELISA法检测小鼠样本中的脂多糖(LPS)水平,具有较高的检测灵敏度,最低检测限可达 0.051 ng/mL,能够满足低丰度样本的检测需求。试剂盒检测范围为 0.16–10 ng/mL,线性范围适中,可覆盖多数生物样本中LPS的浓度变化区间。同时,该试剂盒适用于血清、血浆、组织匀浆、细胞裂解液及细胞培养上清等多种样本类型,具有较好的适用性和兼容性。

- Yuxin Bi首都医科大学 / 心律失常
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Applications: ELISA|Reactivity: Mouse

This kit is primarily used to detect IL-6 expression levels in mouse samples and is well suited for sample types such as cell culture supernatants. During actual use, the operating procedures are clear, the reagents are complete, the standard curve shows good linearity, and the variation between duplicate wells is small. The assay results effectively reflect the changes in IL-6 levels among different treatment groups, providing good reference value for evaluating inflammatory responses and alterations in SASP-related factors. Overall, the user experience is positive, and this kit is recommended for ELISA applications.

- ZhuJianguoNanjing Agricultural University / animal

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