Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

SKU: orb774983

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Beta(MIP1b).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 800 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	12.5-800 pg/mL
Sensitivity	5.4 pg/mL
Concentration	800 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MIP1 Beta,CCL4 ELISA Kit, ACT2 ELISA Kit, G-26 ELISA Kit, LAG1 ELISA Kit, MIP1-B ELISA Kit, SCYA4 ELISA Kit, Chemokine C-C-Motif Ligand 4 ELISA Kit, Small Inducible Cytokine A4 ELISA Kit, Homologous To Mouse Mip-1b ELISA Kit

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Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

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UniProt

P14097

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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