Mouse Maresin 1 (MaR1) ELISA Kit

SKU: orb1817289

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MaR1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MaR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MaR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Application Notes

standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MaR1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MaR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MaR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	15.63-1000 pg/mL
Sensitivity	6.06 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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7(R)-Maresin 1; quinolin-7-ol; 7-hydroxychinoline; (4Z,7R,8E,10E,12Z,14S,16Z,19Z)-7,14-Dihydroxy-4,8,10,12,16,19-docosahexaenoic acid; 7-Chinolinol; 7R,14S-dihydroxydocosa-4Z,8E,10E,12Z,16Z,19Z-hexaenoic acid; 4,8,10,12,16,19-Docosahexaenoic acid, 7,14-dihydroxy-, (4Z,7R,8E,10E,12Z,14S,16Z,19Z)-; 7-Quinolinol; 7-HYDROXYQUINOLINE; 7R-maresin-1; 7-hydoxyquinoline

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