(Mouse) Med15 Antibody (C-term)

SKU: orb1926552

Description

Images & Validation

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Item 1 of 3

Tested Applications	IHC-P, WB
Dilution range	IHC-P - 1:100-500, WB - 1:2000
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	86607 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Alternative Names

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Pcqap

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Staining (Mouse) Med15 in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-Med15 Antibody (C-term) at 1:1000 dilution + mouse testis lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 87 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Med15 Antibody (C-term) at 1:2000 dilution. Lane 1: mouse brain lysates. Lane 2: mouse spleen lysates. Lane 3: mouse thymus lysates. Lane 4: rat lung lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 87 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q924H2

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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