Mouse Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

SKU: orb780844

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related(MCL1).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MCL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.062 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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EAT ELISA Kit, MCL1L ELISA Kit, MCL1S ELISA Kit, BCL2L3 ELISA Kit, TM ELISA Kit, Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 ELISA Kit, Bcl-2-like protein 3 ELISA Kit, Bcl-2-related protein EAT/mcl1 ELISA Kit, mcl1/EAT ELISA Kit

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Mouse Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

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UniProt

P97287

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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