Mouse NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) ELISA Kit

SKU: orb782216

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR Family, Pyrin Domain Containing Protein 3(NLRP3).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NLRP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NLRP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NLRP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.052 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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AVP ELISA Kit, AGTAVPRL ELISA Kit, AII ELISA Kit, AII/AVP ELISA Kit, C1orf7 ELISA Kit, CIAS1 ELISA Kit, CLR1.1 ELISA Kit, FCAS ELISA Kit, FCU ELISA Kit, MWS ELISA Kit, NALP3 ELISA Kit, PYPAF1 ELISA Kit, Cryopyrin ELISA Kit, Cold Induced Autoinflammatory Syndrome 1 ELISA Kit, Caterpiller-Like Receptor 1.1 ELISA Kit

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Mouse NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) ELISA Kit

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UniProt

Q8R4B8

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (3)

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Mouse (3)

Hala A Ahmed 1, Ahmed A Shaaban 2, Mirhan N Makled 3, Tarek M Ibrahim G protein-coupled estrogen receptor selective agonist, G1, improves the molecular and biochemical markers in a cisplatin mouse model of CKD Chem Biol Interact ., (2024)

PubMed: 38795875

Applications

ELISA

Reactivity

Mouse

Sara H Hazem 1, Karim M Saad 2, Mahmoud M Samaha Protective effects of BTK inhibition by acalabrutinib on cisplatin-induced renal and testicular injury in mice: Modulation of mTOR/AMPK, NLRP3/GSDMD-N, and apoptotic pathways Int Immunopharmacol ., (2025)

PubMed: 39938312

Applications

ELISA

Reactivity

Mouse

Fahad O Alenezi 1, Manar A Nader 2, Dalia H El-Kashef 2, Marwa E Abdelmageed Dapansutrile mitigates concanavalin A- induced autoimmune hepatitis: Involvement of NLRP3/IL-1β and JNK/ p38 MAPK pathways Biomed Pharmacother, (2025)

PubMed: 40164046

Applications

ELISA

Reactivity

Mouse