Mouse PDGFRA protein

SKU: orb707264

Description

Recombinant Mouse PDGFR alpha Protein is produced by mammalian expression system and the target gene encoding Leu25-Glu524 is expressed with a Fc tag at the C-terminus.

Research Area

Cancer, Developmental Biology, Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Target	PDGFRA
Reactivity	Mouse
Protein Sequence	LLLPSILPNE NEKIVQLNSS FSLRCVGESE VSWQHPMSEE DDPNVEIRSE ENNSGLFVTV LEVVNASAAH TGWYTCYYNH TQTDESEIEG RHIYIYVPDP DMAFVPLGMT DSLVIVEEDD SAIIPCRTTD PETQVTLHNN GRLVPASYDS RQGFNGTFSV GPYICEATVK GRTFKTSEFN VYALKATSEL NLEMDARQTV YKAGETIVVT CAVFNNEVVD LQWTYPGEVR NKGITMLEEI KLPSIKLVYT LTVPKATVKD SGEYECAARQ ATKEVKEMKR VTISVHEKGF VEIEPTFGQL EAVNLHEVRE FVVEVQAYPT PRISWLKDNL TLIENLTEIT TDVQKSQETR YQSKLKLIRA KEEDSGHYTI IVQNEDDVKS YTFELSTLVP ASILDLVDDH HGSGGGQTVR CTAEGTPLPE IDWMICKHIK KCNNDTSWTV LASNVSNIIT ELPRRGRSTV EGRVSFAKVE ETIAVRCLAK NNLSVVAREL KLVAPTLRSE VDDIEGRMDE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PDGFR2; RHEPDGFRA; Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2; CD140a

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Quick Database Links

Gene Symbol

PDGFRA

UniProt

P26618

NCBI Gene ID

18595

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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