Mouse Peptide YY (PYY) ELISA Kit

SKU: orb777223

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Description

The Mouse Peptide YY (PYY) ELISA Kit is designed to quantify Peptide YY (PYY) in mouse serum, plasma, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 4.66 pg/mL.

Research Area

Gastroenterology & Hepatology, Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PYY. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PYY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PYY in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	15.63-1000 pg/mL
Sensitivity	4.66 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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PYY1 ELISA Kit, Peptide tyrosine tyrosine ELISA Kit

Quality Guarantee

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Quick Database Links

Gene Symbol

PYY

UniProt

Q9EPS2

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Reactivity:	Mouse
Dynamic Range:	15.63-1000pg/mL
Sensitivity:	9.38 pg/mL

Reactivity:	Mouse
Dynamic Range:	15.625-1000pg/ml
Sensitivity:	9.375pg/ml

Reactivity:	Mouse
Dynamic Range:	15.63-1000pg/mL
Sensitivity:	8.84pg/mL

Reactivity:	Mouse
Dynamic Range:	15.63-1000pg/mL
Sensitivity:	8.3pg/mL

Reactivity:	Mouse
Dynamic Range:	15.6-1000 pg/mL
Sensitivity:	4.9 pg/mL