Mouse Perforin 1 (PRF1) ELISA Kit

**Detailed user evaluation of the Mouse Perforin 1 (PRF1) ELISA Kit (Catalog No. orb776932):** **Experimental Applications and Sample Detection** This experiment was mainly used for the quantitative detection of mouse PRF1. The sample types included serum, EDTA plasma, and cell culture supernatants. During the experiment, serum and plasma samples were pre-diluted at least 1:2 according to the recommended protocol, which helped reduce matrix interference and ensured accurate results. **Product Performance** **Detection performance:** The kit showed a sensitivity of 0.062 ng/mL, with a standard curve range of 0.156–10 ng/mL. The sample concentrations measured in this experiment were within this range, and the standard curve showed good linearity. Spike recovery and dilution linearity tests were both within the acceptable range (80%–120%), indicating that the kit can accurately reflect PRF1 concentrations in biological samples. **Reproducibility and stability:** The intra-assay and inter-assay coefficients of variation (CV%) were both below 10%, demonstrating high precision and good repeatability within and between assays. The kit has a shelf life of up to 12 months when stored at 2–8°C, showing satisfactory stability. **User experience:** The kit uses a standard double-antibody sandwich ELISA method, with a total assay time of approximately 3.5 hours. The workflow was smooth and straightforward. The kit provides a pre-coated, detachable 96-well plate, allowing the number of wells used to be adjusted according to sample quantity and reducing waste. However, it should be noted that the standard is provided as a lyophilized powder and must be used within 12 hours after reconstitution. Repeated freeze-thaw cycles should be avoided. **Summary** Overall, this ELISA kit showed good specificity and high sensitivity, meeting the requirements for quantitative detection of mouse PRF1. It is a useful tool for researchers studying cellular immunity, inflammatory responses, and related biological processes.