Mouse Peroxisome Proliferator Activated Receptor Gamma (PPAR-γ) ELISA Kit

SKU: orb775497

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma(PPARg).

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.063 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PPAR-G ELISA Kit, PPARG1 ELISA Kit, PPARG2 ELISA Kit, NR1C3 ELISA Kit, Glitazone Receptor ELISA Kit, Nuclear Receptor Subfamily 1 Group C Member 3 ELISA Kit

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Mouse Peroxisome Proliferator Activated Receptor Gamma (PPAR-γ) ELISA Kit

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UniProt

P37238

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (2)

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Mouse (2)

Runhong Zhou 1, Ruifeng Huang 2, Shaofen Zhou 2, Shengsheng Lu 2, Haixing Lin 2, Jingnan Qiu 2, Shuaiqi Ma 2, Jian He Sorbicillinoid HSL-2 inhibits the infection of influenza A virus via interaction with the PPAR-γ/NF-κB pathway J Infect Chemother, (2024)

PubMed: 38942291

Applications

ELISA

Reactivity

Mouse

Haiwei Ni # 1, Zachary J Reitman # 2, Wei Zou 1, Md Naushad Akhtar 1, Ritama Paul 1, Menggui Huang 1, Duo Zhang 1, Hao Zheng 1, Ruitao Zhang 1, Ruiying Ma 1, Gina Ngo 1, Lin Zhang 3, Eric S Diffenderfer 1, S Azar Oliaei Motlagh 1, Michele M Kim 1, Andy J Minn 1 4, Jay F Dorsey 1, Jessica B Foster 5, James Metz 1, Constantinos Koumenis 1 4, David G Kirsch 6 7 8 9, Yanqing Gong 10, Yi Fan FLASH radiation reprograms lipid metabolism and macrophage immunity and sensitizes medulloblastoma to CAR-T cell therapy Nat Cancer ., (2025)

PubMed: 39910249

Applications

ELISA

Reactivity

Mouse