Mouse Prokineticin 2 (PK2) ELISA Kit

SKU: orb776681

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prokineticin 2(PK2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prokineticin 2(PK2).

Research Area

Neuroscience

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Application Notes

standard: 20000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PK2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PK2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	312.5-20000 pg/mL
Sensitivity	94.4 pg/mL
Concentration	20000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PK-2 ELISA Kit, Bv8 ELISA Kit, MIT1 ELISA Kit, KAL4 ELISA Kit, Protein Bv8 Homolog ELISA Kit

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Q9QXU7

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