Mouse PYD and CARD Domain Containing Protein (PYCARD) ELISA Kit

SKU: orb2667425

Description

Research Area

Cell Biology

Images & Validation

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Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PYCARD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PYCARD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PYCARD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PYCARD in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	7.1 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1

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Quick Database Links

UniProt

Q9EPB4

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No UniProt data available

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