Mouse Sphingosine 1 Phosphate Receptor 3 (S1PR3) ELISA Kit

SKU: orb1947055

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Description

Mouse Sphingosine 1 Phosphate Receptor 3 (S1PR3) ELISA Kit

Research Area

Metabolism Research

Images & Validation

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Application Notes

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse S1PR3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S1PR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S1PR3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse S1PR3. You can calculate the concentration of Mouse S1PR3 in the samples by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Sandwich
Assay Time	3.5H
Range	62.5-4000pg/mL
Sensitivity	37.5 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Sphingosine 1-phosphate receptor 3,S1PR3,S1P receptor 3,S1P3,Endothelial differentiation G-protein coupled receptor 3,Sphingosine 1-phosphate receptor Edg-3,S1P receptor Edg-3,EDG3

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Mouse Sphingosine 1 Phosphate Receptor 3 (S1PR3) ELISA Kit

Quick Database Links

Gene Symbol

S1PR3

UniProt

Q9Z0U9

UniProt Details

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No UniProt data available

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