Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit

SKU: orb779980

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 1(S1PR1).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S1PR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S1PR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S1PR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S1PR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.123 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD363 ELISA Kit, EDG1 ELISA Kit, S1-PR1 ELISA Kit, S1P1 ELISA Kit, ECGF1 ELISA Kit, CHEDG1 ELISA Kit, Endothelial differentiation G-protein coupled receptor 1 ELISA Kit, Sphingolipid G-Protein-Coupled Receptor 1 ELISA Kit

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Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit

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UniProt

O08530

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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