Mouse Squamous Cell Carcinoma Antigen 2 (SCCA2) ELISA Kit

SKU: orb776711

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Squamous Cell Carcinoma Antigen 2(SCCA2).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SCCA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SCCA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SCCA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SCCA2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.056 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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SERPINB4 ELISA Kit, PI11 ELISA Kit, SPB4 ELISA Kit, Leupin ELISA Kit, Serpin Peptidase Inhibitor,Clade B,Member 4 ELISA Kit, Peptidase Inhibitor 11 ELISA Kit

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Mouse Squamous Cell Carcinoma Antigen 2 (SCCA2) ELISA Kit

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UniProt

Q9Z2G2

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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