Mouse Stem Cell Factor Receptor (SCFR) ELISA Kit

SKU: orb776389

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor Receptor(SCFR).

Research Area

Cancer Biology, Hematology, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SCFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SCFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SCFR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.062 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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CD117 ELISA Kit, KIT ELISA Kit, C-Kit ELISA Kit, PBT ELISA Kit, p145 c-Kit ELISA Kit, C-Kit Receptor ELISA Kit, Tyrosine-Protein Kinase ELISA Kit, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog ELISA Kit, Piebald Trait ELISA Kit

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Mouse Stem Cell Factor Receptor (SCFR) ELISA Kit

Quick Database Links

UniProt

P05532

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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