Mouse Superoxide Dismutase 1, Soluble (SOD1) ELISA Kit

SKU: orb1946776

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Description

Mouse Superoxide Dismutase 1, Soluble (SOD1) ELISA Kit

Images & Validation

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Item 1 of 1

Application Notes

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse SOD1. During the reaction, Mouse SOD1 in the sample or standard competes with a fixed amount of Mouse SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Competitive
Assay Time	1.5H
Range	0.16-10ng/mL
Sensitivity	0.1 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]

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Mouse Superoxide Dismutase 1, Soluble (SOD1) ELISA Kit

Quick Database Links

Gene Symbol

SOD1

UniProt

P08228

UniProt Details

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No UniProt data available

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