Mouse TNF protein

SKU: orb392581

Description

Recombinant mouse TNF protein is produced by our E. coli expression system and the target gene encoding Asp89-Leu235 is expressed.

Research Area

Immunology & Inflammation

Images & Validation

−

Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

−

Source	E. coli
Target	TNF
Reactivity	Mouse
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Protein Sequence	MDKPVAHVVA NHQVEEQLEW LSQRANALLA NGMDLKDNQL VVPADGLYLV YSQVLFKGQG CPDYVLLTHT VSRFAISYQE KVNLLSAVKS PCPKDTPEGA ELKPWYEPIY LGGVFQLEKG DQLSAEVNLP KYLDFAESGQ VYFGVIAL

Storage & Handling

−

Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μM filtered solution of PBS, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Gene Symbol

TNF

UniProt

P06804

NCBI Gene ID

21926

UniProt Details

−

No UniProt data available

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

View Guide

Western Blot (IB, immunoblot)

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

View Protocol

Find More Protocols