Mouse Wingless Type MMTV Integration Site Family, Member 4 (WNT4) ELISA Kit

SKU: orb781966

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Description

The Mouse Wingless Type MMTV Integration Site Family, Member 4 (WNT4) ELISA Kit uses a sandwich enzyme immunoassay to quantify Wingless Type MMTV Integration Site Family, Member 4 (WNT4) in mouse tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.055 ng/mL.

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse WNT4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse WNT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse WNT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse WNT4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.055 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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MGC123964, Protein Wnt-4, RP23-246F18.1, SERKAL, WNT 4, WNT 4 protein precursor, WNT4

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Quick Database Links

UniProt

P22724

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

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Reactivity:	Mouse
Dynamic Range:	0.156-10 ng/mL
Sensitivity:	0.052 ng/mL

Reactivity:	Mouse
Dynamic Range:	0.156-10 ng/mL
Sensitivity:	0.052 ng/mL