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Recombinant MSH6 Antibody
SKU: orb1151659
Description
Research Area
Cancer, DNA Damage, Epigenetics, Infectious Diseases
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | Rabbit IgG |
| Clone No. | MSH6/6654R |
| Immunogen | A portion of amino acids 1-100 was used as the immunogen for the recombinant MSH6 antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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IHC staining of FFPE human colon carcinoma tissue with recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue from a patient with Lynch syndrome using recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human HCT-116 cell lysate using recombinant MSH6 antibody (clone MSH6/6654R). Expected molecular weight: 120-160 kDa depending on phosphorylation level.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Recombinant MSH6 Antibody (orb1151659)
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