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MTHFD1 Recombinant Mouse Monoclonal Antibody
SKU: orb1173042
Description
Research Area
Amino Acid Metabolism, Oxidative Stress, Vitamins and Minerals
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB=1:500-1000, ICC/IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, Kappa |
| Clone No. | F9D9 |
| Target | MTHFD1 |
| Molecular Weight | 110 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, Glycerol, rAlbumin. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−C1TC_HUMAN; C-1-tetrahydrofolate synthase, cytoplasmic; MTHFC; MTHFD; C1-THF synthase; Epididymis secretory sperm binding protein; C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed; Methylenetetrahydrofolate dehydrogenase; methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1; CIMAH;
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: room, temperature 2h, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: 293, 3: A20, 4: Raw264.7, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 102 kDa, Observed MW: 102 kDa.
Cell line: HEK-293, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173042.
Quick Database Links
Gene Symbol
MTHFD1
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
MTHFD1 Recombinant Mouse Monoclonal Antibody (orb1173042)
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