MUC1 Antibody / Mucin-1 / EMA

SKU: orb2638903

Description

In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1/Mucin-1/Epithelial Marker Antigen/EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the MUC1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, lambda
Clone No.	MUC1/520
Immunogen	Human milk-fat globule membranes (HMFGM) was used as the immunogen for the MUC1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/520).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with MUC1 antibody (MUC1/520).

Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with MUC1 antibody (MUC1/520).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 antibody (MUC1/520).

SDS-PAGE Analysis of Purified, BSA-Free MUC1 Antibody (clone MUC1/520). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human MCF7 cell lysate with MUC1 antibody (clone MUC1/520). This glycoprotein is commonly visualized between 120~500 kDa.

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UniProt

P15941

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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