Recombinant MUC-1 Antibody

SKU: orb2641035

Description

This mAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

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Tested Applications	IHC-P, WB
Dilution Range	Immunohistochemistry (FFPE only; not suitable for frozen tissues): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant MUC-1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2b, kappa
Clone No.	rMUC1/960
Immunogen	Human milk fat globule membranes were used as the immunogen for this recombinant MUC-1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot testing of human MCF7 cell lysate with recombinant MUC-1 antibody (clone rMUC1/960). This glycoprotein is commonly visualized between 120~500 kDa.

IHC testing of FFPE human ovarian carcinoma with recombinant MUC-1 antibody (clone rMUC1/960). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant MUC-1 antibody (clone rMUC1/960) as confirmation of integrity and purity.

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UniProt

P15941

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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