Recombinant Mucin-1 Antibody / Rabbit Monoclonal

SKU: orb2642211

Description

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to MUC1 is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

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Tested Applications	ELISA, IHC-P, WB
Dilution Range	ELISA: 2-5ug/ml (order BSA/sodium azide-free format),Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	Titration of the recombinant Mucin-1 may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	MUC1/2818R
Immunogen	Human milk fat globule membranes were used as the immunogen for this recombinant Mucin-1 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant Mucin-1 Antibody / Rabbit Monoclonal

IHC testing of FFPE human colon with recombinant Mucin-1 antibody (clone MUC1/2818R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human stomach carcinoma with recombinant Mucin-1 antibody (clone MUC1/2818R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Mucin-1 antibody (clone MUC1/2818R) as confirmation of integrity and purity.

Western blot testing of human MCF7 cell lysate with recombinant Mucin-1 antibody (clone MUC1/2818R). This glycoprotein is commonly visualized between 120~500 kDa.

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UniProt

P15941

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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