Recombinant Multi Cytokeratin Antibody / Rabbit Monoclonal

SKU: orb2640914

Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Images & Validation

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Tested Applications	IF, IHC-P
Dilution range	Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	KRT/1877R
Immunogen	A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the recombinant Multi Cytokeratin antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant Multi Cytokeratin Antibody / Rabbit Monoclonal

Immunofluorescent staining of methanol-fixed human MCF7 cells with recombinant Multi Cytokeratin antibody antibody (clone KRT/1877R, green) and Reddot nuclear stain (red).

IHC staining of FFPE human skin with recombinant Multi Cytokeratin antibody (clone KRT/1877R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Multi Cytokeratin antibody (clone KRT/1877R) as confirmation of integrity and purity.

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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