N-Acetyl-Ser-Asp-Lys-Pro

SKU: orb1296494

Description

Acetyl Ser-Asp-Lys-Pro is a bioactive peptide derived from thymosin β4 processing in bone marrow. It functions to inhibit pluripotent hematopoietic stem cell cycle progression at the S-phase. This activity underlies its research applications in studying hematopoiesis and cytoprotection, such as demonstrated in models of Ara-C-induced toxicity.

Research Area

Metabolism Research

Images & Validation

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Key Properties

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CAS Number	127103-11-1
MW	487.5
Purity	99.85% (May vary between batches)
Formula	C₂₀H₃₃N₅O₉
SMILES	C([C@@H](NC([C@H](NC([C@@H](NC(C)=O)CO)=O)CC(O)=O)=O)CCCCN)(=O)N1[C@H](C(O)=O)CCC1
Target	RAAS,Angiotensin-converting Enzyme (ACE)
Solubility	DMSO:10 mM

Bioactivity

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In Vitro

N-Acetyl-Ser-Asp-Lys-Pro (AcSDKP) is a specific substrate for the N-terminal site of ACE and increases five-fold during ACE inhibitor therapy. AcSDKP inhibited the proliferation of isolated cardiac fibroblasts (P<0.05) but significantly stimulated the proliferation of vascular smooth muscle cells. Flow cytometry of rat cardiac fibroblasts treated with AcSDKP showed significant inhibition of cell progression from G0/G1 phase to S phase. In cardiac fibroblasts transfected with a Smad-sensitive luciferase reporter construct, AcSDKP decreased luciferase activity by 55±9.7% (P=0.01). Additionally, AcSDKP decreased phosphorylation and nuclear translocation of Smad2 in cardiac fibroblasts. In conclusion, AcSDKP inhibits the growth of cardiac fibroblasts and TGFbeta1-stimulated phosphorylation of Smad2. Because AcSDKP increases substantially during ACE inhibitor therapy, this suggests a novel pathway independent of angiotensin II by which ACE inhibitors can inhibit cardiac fibrosis.

Storage & Handling

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Storage	keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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N Acetyl Ser Asp Lys Pro, inhibit, NAcetylSerAspLysPro, N-Acetyl-Ser-Asp-Lys-Pro, Inhibitor, Ac-SDKP, ACE, Angiotensin-converting Enzyme (ACE)

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Compound Identifiers

PubChem CID

14181658 ↗

Key Properties

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No computed properties available.

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