N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride

SKU: orb1307996

Description

N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) is a cell-permeable, irreversible inhibitor of trypsin-like serine proteases. It is widely used in vitro to study protease function and has been applied in research models to investigate the role of such enzymes in inflammation and immune responses, including IFN-γ-mediated pathways.

Research Area

Immunology & Inflammation, Metabolism Research, Neuroscience, Protein Biochemistry

Images & Validation

−

Key Properties

−

CAS Number	4272-74-6
MW	369.31
Purity	97.44%
Formula	C₁₄H₂₂Cl₂N₂O₃S
SMILES	O=S(C1=CC=C(C)C=C1)(N[C@H](C(CCl)=O)CCCCN)=O.[H]Cl
Target	Proteasome,Others,Amino Acids and Derivatives,COX
Solubility	DMSO:245 mg/mL (663.4 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (13.54 mM);H2O:95 mg/mL (257.24 mM)

Bioactivity

−

In Vitro

A lower concentration of anti-Fas (10 ng/mL) is used to examine the interaction among the three effectors simultaneously, that is, anti-Fas, TLCK, and IFN-γ. N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride (50 μM) exhibits a small decrease in cell viability. Beyond 50 μM, a dose-dependent decrease in cell viability is observed. IFN-γ slightly reduces cell viability on its own. The addition of anti-Fas (10 ng/mL) results in a slight decrease in cell survival, which is enhanced more than additively in the presence of TLCK, most prominently between 50 and 100 μM. Upon addition of both anti-Fas and IFN-γ, a decrease (≈46%) in cell viability is observed. Moreover, the decrease in cell survival is further enhanced by the addition of higher concentrations of TLCK, 25 μM, and more.

Storage & Handling

−

Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

inhibit, Inhibitor, N alpha Tosyl L lysine chloromethyl ketone hydrochloride, NalphaTosylLlysine chloromethyl ketone hydrochloride, N-alpha-Tosyl-L-lysine_chloromethyl_ketone_hydrochloride, IFN-γ, Trypsin-like proteasomes

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

73093 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols